Upon receipt, this product is warranted to perform according to specifications published in this document. Hamilton Company guarantees the Guard Column Cartridges and Holder to be free of defects in materials and workmanship. The life of your Guard Column Cartridge is dependent upon the cleanliness of your samples and mobile phase filtering the mobile phase extends the cartridge lifetime. The Guard Column prevents particulates and highly adsorptive compounds from reaching the analytical column. The assembly contributes extra-column volume of 10ul for the tubing and 33ul for the packed cartridge. The Guard Column Assembly was designed to provide ease of use while minimizing contribution to chromatographic band broadening. If the cartridge leaks, tighten the thumb screw just until the leaking stops. Pump approximately 2 ml of mobile phase through the guard column to assure that the packing is properly wetted before performing chromatographic injection. Over-tightening may result in distortion of the cartridge and shortening its useful lifetime.ĥ. It is not necessary to apply large amounts of pressure to achieve sealing of the cartridge to 5,000 psi. Finger-tighten the thumb screw until it seats and then an additional eighth turn (maximum). Slide the holder until the column-end header assembly is gently pressed into the cartridge.Ĥ. Gently press the cartridge onto the injector-side header assembly.ģ. Insert the cartridge with the flow arrow pointing toward the column end of the holder housing.

Back out the thumb screw until the face of the header assembly is flush with the inside edge of the holder housing.Ģ.

Knowledge Base (Articles, FAQs, Downloads)Īfter the holder has been installed, a guard column cartridge may be inserted and the guard column put into service.ġ.